6ZSM
Crystal structure of rsGCaMP double mutant Ile80His/Val116Ile in the ON state (non-illuminated)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-25 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 121.540, 121.540, 96.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.460 - 1.950 |
R-factor | 0.1589 |
Rwork | 0.157 |
R-free | 0.19840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ya9 |
RMSD bond length | 0.018 |
RMSD bond angle | 2.166 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.460 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 53375 | 3888 |
<I/σ(I)> | 22.8 | 1.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.3 | 13.8 |
CC(1/2) | 1.000 | 0.674 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 292 | 0.20 M sodium formate, 0.1 M Bis-Tris-Propane buffer pH 8.5, 19% (w/v) PEG 3350 |