6ZSM
Crystal structure of rsGCaMP double mutant Ile80His/Val116Ile in the ON state (non-illuminated)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Green fluorescent protein,Green fluorescent protein,Calmodulin | polymer | 420 | 47325.0 | 1 | UniProt (P42212) UniProt (K4DIE3) | Aequorea victoria (Jellyfish) | |
| 2 | B, C, D, E, F (A) | CALCIUM ION | non-polymer | 40.1 | 5 | Chemie (CA) | |||
| 3 | G, H, I, J, K... (A) | FORMIC ACID | non-polymer | 46.0 | 14 | Chemie (FMT) | |||
| 4 | U, V, W, X, Y (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 5 | Chemie (EDO) | |||
| 5 | Z (A) | water | water | 18.0 | 444 | Chemie (HOH) |
Sequence modifications
A: 62 - 151 (UniProt: P42212)
A: 160 - 302 (UniProt: P42212)
A: 303 - 451 (UniProt: K4DIE3)
| PDB | External Database | Details |
|---|---|---|
| Met 36 | - | initiating methionine |
| Val 37 | - | expression tag |
| Asp 38 | - | expression tag |
| Ser 39 | - | expression tag |
| Ser 40 | - | expression tag |
| Arg 41 | - | expression tag |
| Arg 42 | - | expression tag |
| Lys 43 | - | expression tag |
| Trp 44 | - | expression tag |
| Asn 45 | - | expression tag |
| Lys 46 | - | expression tag |
| Thr 47 | - | expression tag |
| Gly 48 | - | expression tag |
| His 49 | - | expression tag |
| Ala 50 | - | expression tag |
| Val 51 | - | expression tag |
| Arg 52 | - | expression tag |
| Ala 53 | - | expression tag |
| Ile 54 | - | expression tag |
| Gly 55 | - | expression tag |
| Arg 56 | - | expression tag |
| Leu 57 | - | expression tag |
| Ser 58 | - | expression tag |
| Ser 59 | - | expression tag |
| Leu 60 | - | expression tag |
| Glu 61 | - | expression tag |
| Lys 66 | Met 153 | conflict |
| Ala 76 | Val 163 | conflict |
| His 80 | Ile 167 | conflict |
| Gly 88 | Ser 175 | conflict |
| Tyr 93 | Asp 180 | conflict |
| Ile 116 | Thr 203 | conflict |
| Lys 119 | Ala 206 | conflict |
| Leu 144 | His 231 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly 152 | - | linker |
| Gly 153 | - | linker |
| Thr 154 | - | linker |
| Gly 155 | - | linker |
| Gly 156 | - | linker |
| Ser 157 | - | linker |
| Met 158 | - | linker |
| Val 159 | - | linker |
| - | Phe 64 | deletion |
| - | Ser 65 | deletion |
| Leu 222 | Tyr 66 | conflict |
| Pia 224 | Gly 67 | conflict |
| Leu 227 | Gln 69 | conflict |
| Ile 251 | Val 93 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Thr 373 | Ile 354 | conflict |
| Tyr 381 | Asp 362 | conflict |
| Leu 452 | - | expression tag |
| Glu 453 | - | expression tag |
| His 454 | - | expression tag |
| His 455 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 47325.0 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 1155.1 | |
| All* | Total formula weight | 48480.1 |
