6YA9

Crystal structure of rsGCaMP in the ON state (non-illuminated)

Summary for 6YA9

• Entry DOI: 10.2210/pdb6ya9/pdb
• Descriptor: rsCGaMP, CALCIUM ION (3 entities in total)
• Functional Keywords: reversible switchable fluorescent protein, calcium sensor, calmodulin, fluorescent protein
• Biological source: Aequorea victoria
• Total number of polymer chains: 1
• Total formula weight: 47566.52

Authors

Janowski, R.,Fuenzalida-Werner, J.P.,Mishra, K.,Stiel, A.C.,Niessing, D. (deposition date: 2020-03-11, release date: 2021-10-06, Last modification date: 2024-01-24)

Primary citation

Mishra, K.,Fuenzalida-Werner, J.P.,Pennacchietti, F.,Janowski, R.,Chmyrov, A.,Huang, Y.,Zakian, C.,Klemm, U.,Testa, I.,Niessing, D.,Ntziachristos, V.,Stiel, A.C.
Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging.
Nat.Biotechnol., 2021

PubMed Abstract: Reversibly photo-switchable proteins are essential for many super-resolution fluorescence microscopic and optoacoustic imaging methods. However, they have yet to be used as sensors that measure the distribution of specific analytes at the nanoscale or in the tissues of live animals. Here we constructed the prototype of a photo-switchable Ca sensor based on GCaMP5G that can be switched with 405/488-nm light and describe its molecular mechanisms at the structural level, including the importance of the interaction of the core barrel structure of the fluorescent protein with the Ca receptor moiety. We demonstrate super-resolution imaging of Ca concentration in cultured cells and optoacoustic Ca imaging in implanted tumor cells in mice under controlled Ca conditions. Finally, we show the generalizability of the concept by constructing examples of photo-switching maltose and dopamine sensors based on periplasmatic binding protein and G-protein-coupled receptor-based sensors.

PubMed: 34845372
DOI: 10.1038/s41587-021-01100-5

Experimental method

X-RAY DIFFRACTION (2.15 Å)