6ZG9
Structure of M1-StaR-T4L in complex with GSK1034702 at 2.5A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.96860 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.363, 66.571, 153.102 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.970 - 2.500 |
R-factor | 0.205 |
Rwork | 0.203 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y00 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.950 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.551 | 2.627 |
High resolution limit [Å] | 2.500 | 2.500 |
Rpim | 0.198 | 1.857 |
Number of reflections | 14062 | 506 |
<I/σ(I)> | 4.8 | |
Completeness [%] | 91.2 | |
Redundancy | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 0.1 NaHEPES pH 7.4-7.8, 0.1M di-ammonium hydrogenohosphate, 30-38% PEG300 |