6ZC8
Small-molecule inhibitors of the PDZ domain of Dishevelled proteins interrupt Wnt signalling
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-05-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 89.345, 89.345, 131.659 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.350 - 2.760 |
| R-factor | 0.2465 |
| Rwork | 0.244 |
| R-free | 0.28480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f0a |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.083 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.350 | 2.830 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Rmeas | 0.014 | 0.083 |
| Number of reflections | 8495 | 607 |
| <I/σ(I)> | 21.4 | 4.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.5 M ammonium sulphate, 12% glycerol, 0.1 M Tris-HCl pH 8.5 |
