6Z86
human GTP cyclohydrolase I in complex with 7-deaza-GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.999880 |
| Spacegroup name | P 1 |
| Unit cell lengths | 87.861, 88.595, 163.567 |
| Unit cell angles | 85.22, 88.91, 83.55 |
Refinement procedure
| Resolution | 162.990 - 2.206 |
| R-factor | 0.1832 |
| Rwork | 0.183 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fb1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.739 | 2.457 |
| High resolution limit [Å] | 2.206 | 2.206 |
| Rmerge | 0.045 | 0.337 |
| Number of reflections | 142765 | 7139 |
| <I/σ(I)> | 6.2 | 1.5 |
| Completeness [%] | 89.7 | 62.4 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 0.1 M Morpheus Buffer 1, 30 % EDO_P8K and Morpheus ethylene glycols (Morpheus HT-96; E10; Molecular Dimensions) |
