6Z6B

Structure of full-length La Crosse virus L protein (polymerase)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-26
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9724
Spacegroup name	C 1 2 1
Unit cell lengths	371.187, 145.320, 234.193
Unit cell angles	90.00, 116.33, 90.00

Refinement procedure

Resolution	209.891 - 3.961
Rwork	0.264
R-free	0.29880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5amr
RMSD bond length	0.002
RMSD bond angle	1.156
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	209.891	4.350
High resolution limit [Å]	3.960	3.960
Rmeas	0.163	1.285
Number of reflections	59211	2961
<I/σ(I)>	6.5
Completeness [%]	60.8
Redundancy	11.6
CC(1/2)	0.995	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	LACV-L in complex with pre-annealed 3 prime (1-16) and 5 prime (9-16) vRNA was concentrated to 5 mg/ml. The 5 prime (1-10) vRNA end was later soaked into crystals in 1 to 2 molar ratio. Mother liquor was 100 mM Tris pH 8.0, 100 mM NaCl, and 8% PEG 4000. Crystals were soaked in a stepwise manner with increasing concentration of the glycerol cryo-protectant, reaching 30%