6YIH

Structure of Chromosomal Passenger Complex (CPC) bound to phosphorylated Histone 3 peptide at 2.6 A.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	110
Detector technology	CCD
Collection date	2017-11-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97625
Spacegroup name	P 65
Unit cell lengths	99.365, 99.365, 56.224
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.070 - 2.550
R-factor	0.235
Rwork	0.234
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qfa
RMSD bond length	0.004
RMSD bond angle	0.639
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.220	2.660
High resolution limit [Å]	2.550	2.550
Rmerge	0.091	0.774
Number of reflections	9950	1236
<I/σ(I)>	9.2	1.8
Completeness [%]	96.7
Redundancy	4.5
CC(1/2)	0.992	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	20 % (w/v) PEG 4,000, 100 mM HEPES pH 7.0, 150 mM ammonium sulphate