6YCS

Human Transcription Cofactor PC4 DNA-binding domain in complex with full phosphorothioate 5-10-5 2'-O-methyl DNA gapmer antisense oligonucleotide.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 43 21 2
Unit cell lengths	102.143, 102.143, 83.813
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.680 - 3.050
R-factor	0.2415
Rwork	0.238
R-free	0.31750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c62
RMSD bond length	0.003
RMSD bond angle	0.826
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.680	3.230
High resolution limit [Å]	3.050	3.050
Rmerge	0.184
Number of reflections	8900	1401
<I/σ(I)>	17.76	1.92
Completeness [%]	99.8	99.8
Redundancy	25
CC(1/2)	1.000	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.2 M LiSO4, 0.1 M BIS-TRIS pH 6.5, 21% PEG 3350