6YCS
Human Transcription Cofactor PC4 DNA-binding domain in complex with full phosphorothioate 5-10-5 2'-O-methyl DNA gapmer antisense oligonucleotide.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 102.143, 102.143, 83.813 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.680 - 3.050 |
R-factor | 0.2415 |
Rwork | 0.238 |
R-free | 0.31750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c62 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.826 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.680 | 3.230 |
High resolution limit [Å] | 3.050 | 3.050 |
Rmerge | 0.184 | |
Number of reflections | 8900 | 1401 |
<I/σ(I)> | 17.76 | 1.92 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 25 | |
CC(1/2) | 1.000 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.2 M LiSO4, 0.1 M BIS-TRIS pH 6.5, 21% PEG 3350 |