6Y73

The crystal structure of human MACROD2 in space group P43

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-09
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.91589
Spacegroup name	P 43
Unit cell lengths	96.780, 96.780, 261.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.390 - 1.700
R-factor	0.1831
Rwork	0.182
R-free	0.20310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4iqy
RMSD bond length	0.006
RMSD bond angle	1.352
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.390	48.390	1.740
High resolution limit [Å]	1.700	7.600	1.700
Rmerge	0.123	0.080	1.150
Rmeas	0.133	0.085	1.242
Total number of observations	1823516
Number of reflections	261897	2973	19354
<I/σ(I)>	8.98	23.02	1.41
Completeness [%]	100.0	99.6	100
Redundancy	6.963	7.491	7.063
CC(1/2)	0.995	0.995	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	0.2 M Ammonium tartarate dibasic pH 6.7, 20% PEG 3350