6Y6C

TREM2 extracellular domain (19-174) in complex with single-chain variable fragment (scFv-4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92
Spacegroup name	P 41 2 2
Unit cell lengths	111.661, 111.661, 232.196
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.630 - 2.260
R-factor	0.1924
Rwork	0.191
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5UD7 6G8R
RMSD bond length	0.002
RMSD bond angle	0.497
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.630	2.341
High resolution limit [Å]	2.260	2.260
Rmerge	0.213
Rmeas	0.218
Number of reflections	69496	6791
<I/σ(I)>	9.96
Completeness [%]	99.8
Redundancy	21.4
CC(1/2)	0.999	0.694

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	0.2M potassium chloride, 35% pentaerythritol propoxylate 5/4, 0.1M HEPES pH 7.5