6Y32

Structure of the GTPase heterodimer of human SRP54 and SRalpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.072
Spacegroup name	P 1 2 1
Unit cell lengths	155.825, 70.634, 172.573
Unit cell angles	90.00, 116.81, 90.00

Refinement procedure

Resolution	49.553 - 2.600
R-factor	0.1543
Rwork	0.149
R-free	0.18440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l3q
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.553	2.490
High resolution limit [Å]	2.400	2.400
Rpim	0.062	0.624
Number of reflections	130686	12547
<I/σ(I)>	11.34
Completeness [%]	99.6
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.4 M lithium sulfate, 13% (w/v) PEG 3350, 0.1 M Tris (pH 8.0)