6XK9
Cereblon in complex with DDB1, CC-90009, and GSPT1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97741 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 157.616, 112.041, 176.582 |
Unit cell angles | 90.00, 95.79, 90.00 |
Refinement procedure
Resolution | 74.450 - 3.640 |
R-factor | 0.2097 |
Rwork | 0.208 |
R-free | 0.24550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hxb |
RMSD bond length | 0.003 |
RMSD bond angle | 0.673 |
Data reduction software | xia2 |
Data scaling software | DIALS |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.450 | 3.770 |
High resolution limit [Å] | 3.640 | 3.640 |
Rmerge | 0.118 | 0.471 |
Rmeas | 0.142 | 0.564 |
Rpim | 0.077 | 0.308 |
Number of reflections | 68917 | 6809 |
<I/σ(I)> | 4.49 | 2.33 |
Completeness [%] | 99.8 | |
Redundancy | 3.3 | |
CC(1/2) | 0.992 | 0.696 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% PEG 3350, 100mM Tris-HCl (pH 7.5), 300mM sodium citrate |