6XAG
Apo BRAF dimer bound to 14-3-3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-10-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97920 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.504, 115.752, 153.092 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 92.330 - 3.300 |
R-factor | 0.2518 |
Rwork | 0.250 |
R-free | 0.29970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6u2h |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1-3660_final) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.330 | 3.418 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmeas | 0.181 | 0.992 |
Number of reflections | 16106 | 3218 |
<I/σ(I)> | 8.13 | 1.14 |
Completeness [%] | 94.3 | 98.38 |
Redundancy | 2 | |
CC(1/2) | 0.998 | 0.526 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 100 mM HEPES pH 7.5, 200 mM ammonium sulfate, 23% (w/v) PEG 3350 |