6XAF
1.9A crystal structure of the GTPase domain of Parkinson's disease-associated protein LRRK2 carrying R1398H
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.497, 101.785, 44.527 |
Unit cell angles | 90.00, 101.38, 90.00 |
Refinement procedure
Resolution | 20.048 - 1.968 |
R-factor | 0.1834 |
Rwork | 0.180 |
R-free | 0.22140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ojf |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.050 | 1.990 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmeas | 0.115 | |
Rpim | 0.062 | |
Number of reflections | 27384 | 2565 |
<I/σ(I)> | 13.64 | |
Completeness [%] | 99.3 | |
Redundancy | 3.3 | |
CC(1/2) | 0.968 | 0.862 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.5 | 298 | 100 mM potassium thiocyanate, 25% PEG2000 MME, 0.1 M Bis-Tris, pH 6.5 |