6X3L
Sortilin-Progranulin Interaction With Compound 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-04 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.832, 75.513, 110.984 |
| Unit cell angles | 90.00, 127.01, 90.00 |
Refinement procedure
| Resolution | 46.240 - 2.700 |
| R-factor | 0.2194 |
| Rwork | 0.217 |
| R-free | 0.26890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f6k |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.240 | 2.900 |
| High resolution limit [Å] | 2.700 | 2.890 |
| Rmerge | 0.036 | 0.271 |
| Number of reflections | 29077 | 187 |
| <I/σ(I)> | 15.8 | |
| Completeness [%] | 98.4 | |
| Redundancy | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20-25% PEG3350, 0.4M Na Malonate, pH8, 0.1M Tris, 5% Glycerol |
