6WVZ

Crystal structure of anti-MET Fab arm of amivantamab in complex with human MET

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	86.830, 86.830, 457.099
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.450 - 3.100
R-factor	0.192
Rwork	0.190
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1SHY and 4LEO
RMSD bond length	0.003
RMSD bond angle	0.875
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.450	3.150
High resolution limit [Å]	3.100	3.100
Rmerge	0.110	0.431
Rmeas	0.121	0.060
Rpim	0.049	0.024
Number of reflections	31529	1906
<I/σ(I)>	7.2	2.4
Completeness [%]	94.9	96.7
Redundancy	5.1	4.5
CC(1/2)		0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	2.5M sodium formate, 5% PEG 400, 0.1M Tris pH 8.5