6WVW

Crystal structure of the R59P-SNAP25 containing SNARE complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	48.761, 45.899, 109.066
Unit cell angles	90.00, 94.41, 90.00

Refinement procedure

Resolution	42.290 - 2.110
Rwork	0.200
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n7s
RMSD bond length	0.005
RMSD bond angle	0.680
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.290	2.186
High resolution limit [Å]	2.110	2.110
Rmerge	0.155
Number of reflections	19820	498
<I/σ(I)>	11.2
Completeness [%]	89.6
Redundancy	11.4
CC(1/2)	0.999	0.374

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	100 mM MES (pH 6.0), 100 mM CaCl2, 20-25% (v/v)