6WVW

Crystal structure of the R59P-SNAP25 containing SNARE complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Vesicle-associated membrane protein 2	polymer	63	7231.1	2	UniProt (P63045) Pfam (PF00957) In PDB	Rattus norvegicus (Rat)	VAMP-2,Synaptobrevin-2
2	B, F	Syntaxin-1A	polymer	66	7706.8	2	UniProt (P32851) Pfam (PF05739) In PDB	Rattus norvegicus (Rat)	Neuron-specific antigen HPC-1,Synaptotagmin-associated 35 kDa protein,P35A
3	C, G	Synaptosomal-associated protein 25	polymer	74	8567.5	2	UniProt (P60881) In PDB	Rattus norvegicus (Rat)	SNAP-25,Super protein,SUP,Synaptosomal-associated 25 kDa protein
4	D, H	Synaptosomal-associated protein 25	polymer	64	7340.2	2	UniProt (P60881) In PDB	Rattus norvegicus (Rat)	SNAP-25,Super protein,SUP,Synaptosomal-associated 25 kDa protein
5	B, C, E, F	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
6	C, E	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	2	Chemie (MPD)
7		water	water		18.0	264	Chemie (HOH)

Sequence modifications

A, E: 28 - 89 (UniProt: P63045)

PDB	External Database	Details
Gly 27	-	expression tag

C, G: 10 - 83 (UniProt: P60881)

PDB	External Database	Details
Pro 59	Arg 59	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	61691.1
Non-Polymers*	Number of molecules	6
	Total formula weight	396.7
All*	Total formula weight	62087.7

*Water molecules are not included.