6WDQ
IL23/IL23R/IL12Rb1 signaling complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-05-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 75.712, 118.483, 186.151 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.990 - 3.400 |
R-factor | 0.243 |
Rwork | 0.238 |
R-free | 0.29460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mzv D_1000248020 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.557 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.990 | 3.522 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.072 | 2.118 |
Rmeas | 0.089 | 2.602 |
Rpim | 0.051 | 1.484 |
Number of reflections | 21806 | 2240 |
<I/σ(I)> | 8.59 | 0.57 |
Completeness [%] | 89.7 | 75.95 |
Redundancy | 2.7 | 2.7 |
CC(1/2) | 0.999 | 0.286 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 9.4 | 295 | PEG 12000 CAPSO pH 9.4 5% dioxane 10% spermine HCl |