6WCD

Crystal Structure of Xenopus laevis APE2 Catalytic Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	105
Detector technology	CCD
Collection date	2014-02-01
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	56.383, 73.149, 98.877
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.170 - 1.540
R-factor	0.144
Rwork	0.143
R-free	0.15700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3g0r
RMSD bond length	0.014
RMSD bond angle	1.346
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.170	1.600
High resolution limit [Å]	1.540	1.540
Rmeas	0.084	0.477
Number of reflections	60632	5767
<I/σ(I)>	16.14
Completeness [%]	99.3	94.99
Redundancy	5.3	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	25% PEG 6000, 0.1 M MES pH 6.5, 2:1 and 1:1 protein:crystallant ratios