6WC2

Crystal Structure of a Ternary MEF2 Chimera/NKX2-5/myocardin enhancer DNA Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-02-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	69.370, 133.900, 140.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.120 - 2.100
R-factor	0.1876
Rwork	0.186
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6byy
RMSD bond length	0.012
RMSD bond angle	1.858
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.000	2.175
High resolution limit [Å]	2.100	2.100
Number of reflections	76673	7471
<I/σ(I)>	12.92
Completeness [%]	99.7
Redundancy	6.5
CC(1/2)	0.997	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.15 M DL-Malic acid pH 7.0, 20% Polyethylene glycol 3350