6WBN
Cryo-EM structure of human Pannexin 1 channel N255A mutant, gap junction
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.83 |
| Number of Particle | 3945051 |
| Software | Gautomatch,RELION,SerialEM,Gctf,Coot,PHENIX |
| Symmetry | C7 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
