6VKP
Crystal structure of DPO4 extension past 8-oxoadenine (oxoA) and dG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2019-07-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.098, 103.690, 99.105 |
Unit cell angles | 90.00, 89.94, 90.00 |
Refinement procedure
Resolution | 49.550 - 2.540 |
Rwork | 0.210 |
R-free | 0.26010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jx4 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.239 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.570 |
High resolution limit [Å] | 2.530 | 2.530 |
Rpim | 0.064 | 0.637 |
Number of reflections | 35565 | 1729 |
<I/σ(I)> | 15.68 | 1.2 |
Completeness [%] | 99.9 | |
Redundancy | 6.3 | |
CC(1/2) | 0.478 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 50 mM Tris 7.5 18% PEG 3350 100 mM magnesium acetate |