6VJD

Estrogen Receptor Alpha Ligand Binding Domain in Complex with Lasofoxifene

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-19
Detector	MAR CCD 165 mm
Wavelength(s)	0.987
Spacegroup name	P 32
Unit cell lengths	58.362, 58.362, 275.621
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.181 - 1.800
R-factor	0.1967
Rwork	0.195
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w9c
RMSD bond length	0.007
RMSD bond angle	0.966
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Rpim	0.071
Number of reflections	89624	4302
<I/σ(I)>	15
Completeness [%]	91.8
Redundancy	4.3
CC(1/2)	0.598	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 3350, Tris pH 7.5, MgCl2