6VEP
Human insulin in complex with the human insulin microreceptor in turn in complex with Fv 83-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.030, 130.120, 148.180 |
| Unit cell angles | 90.00, 90.27, 90.00 |
Refinement procedure
| Resolution | 48.890 - 2.900 |
| R-factor | 0.1949 |
| Rwork | 0.193 |
| R-free | 0.22530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oga |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.263 | 3.640 |
| Number of reflections | 83231 | 4086 |
| <I/σ(I)> | 5.7 | 0.44 |
| Completeness [%] | 99.0 | 97.9 |
| Redundancy | 3.8 | 3.6 |
| CC(1/2) | 0.984 | 0.112 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | Protein: 4.5 mg/ml in 10 mM HEPES pH 7.5 Well condition: 16% PEG 3350, 0.2 M sodium thiocyanate |
