6V2E
Crystal structure of the human CLR:RAMP2 extracellular domain heterodimer with bound high-affinity adrenomedullin S45R/K46L/S48G/Q50W variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2018-12-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.017, 84.993, 123.064 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.970 - 1.830 |
| R-factor | 0.1582 |
| Rwork | 0.157 |
| R-free | 0.18960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rwf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.530 |
| Data reduction software | HKL-2000 (v.712) |
| Data scaling software | HKL-2000 (v.712) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
| High resolution limit [Å] | 1.830 | 4.970 | 1.830 |
| Rmerge | 0.087 | 0.029 | 1.180 |
| Rmeas | 0.093 | 0.031 | 1.274 |
| Rpim | 0.034 | 0.012 | 0.476 |
| Total number of observations | 468938 | ||
| Number of reflections | 63239 | 3416 | 3064 |
| <I/σ(I)> | 6.8 | ||
| Completeness [%] | 99.6 | 99.6 | 98.9 |
| Redundancy | 7.4 | 6.9 | 6.9 |
| CC(1/2) | 0.999 | 0.631 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293 | 20% PEG MME 5000, 0.1 M sodium HEPEs pH 8.2, 150 mM sodium formate, 3% (v/v) dimethyl sulfoxide |
