6V2E
Crystal structure of the human CLR:RAMP2 extracellular domain heterodimer with bound high-affinity adrenomedullin S45R/K46L/S48G/Q50W variant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Maltose/maltodextrin-binding periplasmic protein,Receptor activity-modifying protein 2,Calcitonin gene-related peptide type 1 receptor | polymer | 591 | 66308.4 | 1 | UniProt (P0AEX9) UniProt (O60895) UniProt (Q16602) Pfam (PF01547) Pfam (PF04901) Pfam (PF02793) In PDB | Escherichia coli (strain K12) | MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Calcitonin-receptor-like receptor activity-modifying protein 2,CRLR activity-modifying protein 2,CGRP type 1 receptor,Calcitonin receptor-like receptor |
| 2 | B | ADM | polymer | 16 | 1860.1 | 1 | UniProt (P35318) In PDB | Homo sapiens | |
| 3 | C | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 4 | A | FORMIC ACID | non-polymer | 46.0 | 3 | Chemie (FMT) | |||
| 5 | B | AMINO GROUP | non-polymer | 16.0 | 1 | Chemie (NH2) | |||
| 6 | water | water | 18.0 | 602 | Chemie (HOH) |
Sequence modifications
A: 2 - 368 (UniProt: P0AEX9)
A: 1055 - 2019 (UniProt: O60895)
A: 2029 - 2144 (UniProt: Q16602)
B: 37 - 52 (UniProt: P35318)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Asn 369 | - | linker |
| Ala 370 | - | linker |
| Ala 371 | - | linker |
| Ala 372 | - | linker |
| Glu 373 | - | linker |
| Phe 374 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Arg 1106 | Leu 106 | engineered mutation |
| Gly 2020 | - | linker |
| Ser 2021 | - | linker |
| Ala 2022 | - | linker |
| Gly 2023 | - | linker |
| Ser 2024 | - | linker |
| Ala 2025 | - | linker |
| Gly 2026 | - | linker |
| Ser 2027 | - | linker |
| Ala 2028 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| His 2145 | - | expression tag |
| His 2146 | - | expression tag |
| His 2147 | - | expression tag |
| His 2148 | - | expression tag |
| His 2149 | - | expression tag |
| His 2150 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Arg 45 | Ser 139 | engineered mutation |
| Leu 46 | Lys 140 | engineered mutation |
| Gly 48 | Ser 142 | engineered mutation |
| Trp 50 | Gln 144 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 68168.5 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 342.3 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 154.1 | |
| All* | Total formula weight | 68664.8 |
