6V0O
PRMT5 bound to the PBM peptide from pICln
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 100.010, 136.400, 179.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.940 - 2.860 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gqb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.940 | 2.880 |
| High resolution limit [Å] | 2.860 | 2.860 |
| Rmeas | 0.130 | 2.020 |
| Number of reflections | 28182 | 564 |
| <I/σ(I)> | 8.37 | 0.62 |
| Completeness [%] | 98.1 | 97.1 |
| Redundancy | 3.7 | 3.4 |
| CC(1/2) | 0.997 | 0.481 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 281 | 30% PEG 3350 200 mM ammonium sulfate 0.05% w/v dodecyl-maltoside |
