6URI

HIV-1 Nef in complex with the CD4 cytoplasmic domain and the AP2 clathrin adaptor complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 41
Unit cell lengths	109.154, 109.154, 178.915
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.820 - 3.000
R-factor	0.2419
Rwork	0.241
R-free	0.27680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xa7 4emz
RMSD bond length	0.005
RMSD bond angle	0.875
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.287	0.081
Rmeas	0.296	0.084
Rpim	0.074	0.021
Number of reflections	41865	4189
<I/σ(I)>	11.8	1.4
Completeness [%]	100.0	100
Redundancy	16	15.9
CC(1/2)	0.997	0.366

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.9	298	15% Peg 4000, 0.2M potassium chloride, ~10% 1,6-hexanediol, 0.1M HEPES