6TVO

Human CRM1-RanGTP in complex with Leptomycin B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	180
Detector technology	PIXEL
Collection date	2017-10-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97630
Spacegroup name	I 2 2 2
Unit cell lengths	122.290, 151.330, 234.843
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.910 - 3.201
R-factor	0.2169
Rwork	0.216
R-free	0.23540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nc1
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (V1.15)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	73.910	73.910	3.390
High resolution limit [Å]	3.200	9.560	3.200
Rmerge	0.058	0.016	0.902
Rmeas	0.065	0.018	1.014
Total number of observations	173383
Number of reflections	36131	1464	5638
<I/σ(I)>	17.63	71.05	1.96
Completeness [%]	99.5	98.9	98.8
Redundancy	4.799	4.426	4.885
CC(1/2)	0.999	0.999	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.02M sodium L-glutamate, 0.02M DL-alanine, 0.02M glycine, 0.02M DL-lysine HCl, 0.02 M DL-serine, 10%w/v PEG 8000, 20% v/v ethylene glycol, 0.1M bicine /Trizma base pH 8.5