6TKA

Crystal structure of human O-GlcNAc transferase bound to substrate 7 and a peptide from HCF-1 pro-repeat 2 (11-26)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9159
Spacegroup name	P 32 2 1
Unit cell lengths	100.859, 100.859, 132.629
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	72.950 - 1.910
Rwork	0.184
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n39
RMSD bond length	0.009
RMSD bond angle	1.570
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.320	1.960
High resolution limit [Å]	1.910	1.910
Rmerge	0.100
Rmeas	0.104
Rpim	0.030	0.710
Number of reflections	61015	4473
<I/σ(I)>	15.6	1.1
Completeness [%]	100.0	100
Redundancy	12	12.3
CC(1/2)	1.000	0.405

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.45 M Potassium Sodium Tartrate 0.1 M Tris pH 8.5