6TKA
Crystal structure of human O-GlcNAc transferase bound to substrate 7 and a peptide from HCF-1 pro-repeat 2 (11-26)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-04 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9159 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 100.859, 100.859, 132.629 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.950 - 1.910 |
| Rwork | 0.184 |
| R-free | 0.23320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n39 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.570 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.320 | 1.960 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.100 | |
| Rmeas | 0.104 | |
| Rpim | 0.030 | 0.710 |
| Number of reflections | 61015 | 4473 |
| <I/σ(I)> | 15.6 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12 | 12.3 |
| CC(1/2) | 1.000 | 0.405 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.45 M Potassium Sodium Tartrate 0.1 M Tris pH 8.5 |
