6TKA

Crystal structure of human O-GlcNAc transferase bound to substrate 7 and a peptide from HCF-1 pro-repeat 2 (11-26)

This is a large structure.

Summary for 6TKA

DescriptorUDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, HCF-1 pro-repeat 2 (11-26), [[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{S},6~{R})-3-[6-(butanoylamino)hexanoylamino]-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl] hydrogen phosphate, ... (5 entities in total)
Functional Keywordstransferase complex o-glcnac ogt, transferase
Biological sourceHomo sapiens (Human)
Total number of polymer chains2
Total molecular weight84066.3
Authors
Meek, R.W.,Davies, G.J. (deposition date: 2019-11-28, release date: 2020-03-04)
Primary citation
Alteen, M.G.,Gros, C.,Meek, R.,Cardoso, D.A.,Busmann, J.,Sangouard, G.,Deen, M.C.,Tan, H.Y.,Shen, D.L.,Russell, C.C.,Davies, G.J.,Robinson, P.J.,McCluskey, A.,Vocadlo, D.J.
A direct fluorescent activity assay for glycosyltransferases enables convenient high-throughput screening: Application to O-GlcNAc Transferase.
Angew.Chem.Int.Ed.Engl., 2020
PubMed: 32092778 (PDB entries with the same primary citation)
DOI: 10.1002/anie.202000621
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.91 Å)
?

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.23720 2.1% 0.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload