6TEO

Crystal structure of a yeast Snu114-Prp8 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-16
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.91841
Spacegroup name	C 1 2 1
Unit cell lengths	173.110, 158.590, 110.700
Unit cell angles	90.00, 116.54, 90.00

Refinement procedure

Resolution	47.499 - 3.100
R-factor	0.2324
Rwork	0.230
R-free	0.27140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jb9
RMSD bond length	0.003
RMSD bond angle	0.737
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.180
High resolution limit [Å]	3.100	3.100
Rmerge	0.097	1.517
Rmeas	0.110	1.731
Number of reflections	48253	3522
<I/σ(I)>	12.32	0.516
Completeness [%]	99.3
Redundancy	4.2956
CC(1/2)	0.998	0.516

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295.15	10% (w/v) PEG8000, 200mM Lithium chloride, 50mM magnesium sulfate