6TEO

Crystal structure of a yeast Snu114-Prp8 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Pre-mRNA-splicing factor SNU114	polymer	946	107416.1	2	UniProt (P36048) Pfam (PF16004) Pfam (PF00009) Pfam (PF03764) Pfam (PF00679)	Saccharomyces cerevisiae S288C	114 kDa U5 small nuclear ribonucleoprotein component,Growth inhibitory protein 10
2	B, D (B, D)	Pre-mRNA-splicing factor 8	polymer	250	29357.1	2	UniProt (P33334)	Saccharomyces cerevisiae S288C
3	E, H (A, C)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	F, I (A, C)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
5	G, J (A, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	K, L, M, N (A, B, C, D)	water	water		18.0	5	Chemie (HOH)

Sequence modifications

A, C: 72 - 1008 (UniProt: P36048)

PDB	External Database	Details
Met 71	-	initiating methionine
Trp 1009	-	expression tag
Ser 1010	-	expression tag
His 1011	-	expression tag
Pro 1012	-	expression tag
Gln 1013	-	expression tag
Phe 1014	-	expression tag
Glu 1015	-	expression tag
Lys 1016	-	expression tag

B, D: 315 - 555 (UniProt: P33334)

PDB	External Database	Details
Met 306	-	initiating methionine
Trp 307	-	expression tag
Ser 308	-	expression tag
His 309	-	expression tag
Pro 310	-	expression tag
Gln 311	-	expression tag
Phe 312	-	expression tag
Glu 313	-	expression tag
Lys 314	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	273546.4
Non-Polymers*	Number of molecules	6
	Total formula weight	1287.1
All*	Total formula weight	274833.5

*Water molecules are not included.