6TD3
Structure of DDB1 bound to CR8-engaged CDK12-cyclinK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 250.749, 250.749, 217.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.000 - 3.460 |
| R-factor | 0.1951 |
| Rwork | 0.194 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6h0f 4nst |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.010 |
| Data reduction software | DIALS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7 (3-OCT-2019)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.000 | 3.630 |
| High resolution limit [Å] | 3.460 | 3.460 |
| Rmeas | 0.318 | |
| Rpim | 0.092 | |
| Number of reflections | 89203 | 4460 |
| <I/σ(I)> | 7.2 | 0.9 |
| Completeness [%] | 95.1 | 68.3 |
| Redundancy | 12 | 11.6 |
| CC(1/2) | 0.416 | 0.416 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 0.9 M ammonium citrate tribasic |
