6SWR

Crystal structure of the lysosomal potassium channel MtTMEM175 T38A mutant soaked with zinc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.280961
Spacegroup name	P 21 2 21
Unit cell lengths	129.788, 131.611, 151.771
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.720 - 3.200
Rwork	0.269
R-free	0.29900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1anf 5jqh
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.720	3.320
High resolution limit [Å]	3.200	3.200
Number of reflections	43526	4510
<I/σ(I)>	6.5
Completeness [%]	99.8
Redundancy	51.8
CC(1/2)	0.993	0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Tris-HCl pH 8.5, 150 mM NaCl, 150 mM MgCl2 and 28-30 % PEG400