6SSI
Structure of the pentameric ligand-gated ion channel ELIC in complex with a PAM nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.604, 146.563, 140.242 |
| Unit cell angles | 90.00, 111.39, 90.00 |
Refinement procedure
| Resolution | 47.060 - 2.590 |
| R-factor | 0.231 |
| Rwork | 0.230 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vl0 3p0g |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.940 |
| Data reduction software | XDS (Mar 15, 2019) |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.350 | 49.350 | 2.630 |
| High resolution limit [Å] | 2.580 | 14.160 | 2.580 |
| Rmerge | 0.068 | 0.027 | 1.394 |
| Rmeas | 0.077 | 0.031 | 1.572 |
| Rpim | 0.035 | 0.014 | 0.719 |
| Total number of observations | 594559 | 3177 | 26957 |
| Number of reflections | 123740 | 744 | 5799 |
| <I/σ(I)> | 13.4 | 45 | 1 |
| Completeness [%] | 99.7 | 93.4 | 95.2 |
| Redundancy | 4.8 | 4.3 | 4.6 |
| CC(1/2) | 0.999 | 0.999 | 0.458 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M GABA, 0.2 M Ca(OAc)2, 0.1 M MES buffer, 10% PEG8000 |
