6SSI
Structure of the pentameric ligand-gated ion channel ELIC in complex with a PAM nanobody
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E (A, B, C, D, E) | Cys-loop ligand-gated ion channel | polymer | 318 | 36465.6 | 5 | UniProt (P0C7B7) Pfam (PF02931) | Dickeya chrysanthemi (Pectobacterium chrysanthemi) | ELIC |
| 2 | F, G, H, I, J (F, G, H, I, J) | NANOBODY 22 | polymer | 121 | 13316.7 | 5 | Lama glama | ||
| 3 | BA, HA, K, O, V (D, E, A, B, C) | GAMMA-AMINO-BUTANOIC ACID | non-polymer | 103.1 | 5 | Chemie (ABU) | |||
| 4 | CA, IA, L, P, W (D, E, A, B, C) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 5 | Chemie (MES) | |||
| 5 | DA, JA, KA, M, Q... (D, E, A, B, C) | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
| 6 | FA, MA, N, S, Z (D, E, A, B, C) | CALCIUM ION | non-polymer | 40.1 | 5 | Chemie (CA) | |||
| 7 | EA, LA, R, Y (D, E, B, C) | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 4 | Chemie (PG4) | |||
| 8 | AA, OA, PA, T, U (C, E, B) | UNDECYL-MALTOSIDE | non-polymer | 496.6 | 5 | Chemie (UMQ) | |||
| 9 | GA, NA (D, E) | ACETATE ION | non-polymer | 59.0 | 2 | Chemie (ACT) | |||
| 10 | QA, RA, SA, TA, UA... (A, B, C, D, E...) | water | water | 18.0 | 145 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E: 8 - 321 (UniProt: P0C7B7)
| PDB | External Database | Details |
|---|---|---|
| Gly 5 | - | expression tag |
| Pro 6 | - | expression tag |
| Val 7 | - | expression tag |
| Gly 164 | - | insertion |
| Asn 289 | Met 288 | conflict |
| Gly 322 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 248911.5 | |
| Non-Polymers* | Number of molecules | 32 |
| Total formula weight | 5622.7 | |
| All* | Total formula weight | 254534.2 |
