6SQS
Crystal structure of cat phospho-Ser429 MDM2 RING domain bound to UbcH5B-Ub
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-25 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.916 |
Spacegroup name | P 1 |
Unit cell lengths | 54.593, 56.438, 60.742 |
Unit cell angles | 66.44, 69.44, 89.10 |
Refinement procedure
Resolution | 29.200 - 1.830 |
Rwork | 0.211 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mnj |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.200 | 1.880 |
High resolution limit [Å] | 1.830 | 1.830 |
Number of reflections | 49057 | 3293 |
<I/σ(I)> | 10.2 | |
Completeness [%] | 95.8 | |
Redundancy | 1.8 | |
CC(1/2) | 0.996 | 0.539 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 292 | 0.1 M Tris-HCl, pH 8.0, 15% (w/v) PEG 2000 MME and 0.1 M KCl |