6SQS

Crystal structure of cat phospho-Ser429 MDM2 RING domain bound to UbcH5B-Ub

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	E3 ubiquitin-protein ligase Mdm2	polymer	64	7287.8	2	UniProt (Q7YRZ8) Pfam (PF13920) In PDB	Felis catus (Domestic cat)	Double minute 2 protein,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2
2	B, E	Ubiquitin-conjugating enzyme E2 D2	polymer	146	16720.2	2	UniProt (P62837) Pfam (PF00179) In PDB	Homo sapiens (Human)	(E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1
3	C, F	Ubiquitin-40S ribosomal protein S27a	polymer	77	8663.9	2	UniProt (J3QTR3) Pfam (PF00240) UniProt (by SIFTS) (P62979) In PDB	Homo sapiens (Human)
4	A, D	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5		water	water		18.0	180	Chemie (HOH)

Sequence modifications

A, D: 428 - 491 (UniProt: Q7YRZ8)

PDB	External Database	Details
Thr 443	Gly 443	engineered mutation

B, E: 2 - 147 (UniProt: P62837)

PDB	External Database	Details
Arg 22	Ser 22	engineered mutation
Lys 85	Cys 85	engineered mutation

C, F: 1 - 76 (UniProt: J3QTR3)

PDB	External Database	Details
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	65343.7
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	65605.3

*Water molecules are not included.