6SF2

Ternary complex of human bone morphogenetic protein 9 (BMP9) growth factor domain, its prodomain and extracellular domain of activin receptor-like kinase 1 (ALK1).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9282
Spacegroup name	P 61
Unit cell lengths	72.610, 72.610, 438.485
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	62.250 - 3.300
R-factor	0.2441
Rwork	0.243
R-free	0.27430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fao 4ycg
RMSD bond length	0.002
RMSD bond angle	0.490
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.880	3.480
High resolution limit [Å]	3.300	3.300
Rmerge	0.154
Rmeas	0.163
Rpim	0.052	0.369
Number of reflections	19622	2834
<I/σ(I)>	13.3	2.8
Completeness [%]	100.0	99.9
Redundancy	9.9	10.2
CC(1/2)	0.997	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.14 M potassium sodium tartrate, 14% PEG 3350