6RUV

Structure of the SCIN stabilized C3bBb convertase bound to Properdin and a the non-inhibitory nanobody hFPNb1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	123.990, 354.030, 367.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.819 - 6.150
R-factor	0.2437
Rwork	0.242
R-free	0.27070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2win
RMSD bond length	0.005
RMSD bond angle	0.951
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	6.310
High resolution limit [Å]	6.150	6.150
Number of reflections	38393	2738
<I/σ(I)>	12.2
Completeness [%]	99.6
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	277	100 mM NaCl, 5 % (w/v) PEG4000, 10 mM MgCl2, 100 mM Sodium Cacodylate trihydrate pH 5.8.