6RUV
Structure of the SCIN stabilized C3bBb convertase bound to Properdin and a the non-inhibitory nanobody hFPNb1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | R, S | Nanobody hFPNb1 | polymer | 131 | 14517.2 | 2 | Lama glama | ||
| 2 | U, X | Properdin | polymer | 170 | 18636.9 | 2 | UniProt (P27918) Pfam (PF18487) Pfam (PF00090) In PDB | Homo sapiens (Human) | Complement factor P |
| 3 | V, Y | Properdin | polymer | 221 | 24724.1 | 2 | UniProt (P27918) Pfam (PF00090) Pfam (PF22195) In PDB | Homo sapiens (Human) | Complement factor P |
| 4 | A, G | Complement C3 | polymer | 645 | 71393.3 | 2 | UniProt (P01024) Pfam (PF17790) Pfam (PF01835) Pfam (PF17791) Pfam (PF17789) Pfam (PF07703) In PDB | Homo sapiens (Human) | C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 |
| 5 | B, H | Complement C3 | polymer | 915 | 104073.2 | 2 | UniProt (P01024) Pfam (PF07677) Pfam (PF01759) Pfam (PF00207) Pfam (PF21406) Pfam (PF07678) Pfam (PF21308) In PDB | Homo sapiens (Human) | C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 |
| 6 | J, L | Complement factor B | polymer | 505 | 57042.1 | 2 | UniProt (P00751) Pfam (PF00092) Pfam (PF00089) In PDB | Homo sapiens (Human) | C3/C5 convertase,Glycine-rich beta glycoprotein,GBG,PBF2,Properdin factor B |
| 7 | N, Q | Inhibitor | polymer | 86 | 9876.2 | 2 | UniProt (A0A0H2DUF0) Pfam (PF11546) UniProt (by SIFTS) (Q931M7) In PDB | Staphylococcus aureus | Involved in expression of fibrinogen binding protein,phage associated,Staphylococcal complement inhibitor |
| 8 | C, D, F, I, K | beta-D-glucopyranose-(1-3)-alpha-L-fucopyranose | branched | 326.3 | 5 | In PDB GlyTouCan (G36855WW) | |||
| 9 | E, M | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 570.5 | 2 | In PDB GlyTouCan (G21290RB) | |||
| 10 | a, b, c, O, P... | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 8 | In PDB GlyTouCan (G42666HT) | |||
| 11 | U, V, X, Y | alpha-D-mannopyranose | non-polymer | 180.2 | 21 | Chemie (MAN) | |||
| 12 | J, L | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) |
Sequence modifications
U, X: 28 - 191 (UniProt: P27918)
V, Y: 256 - 469 (UniProt: P27918)
J, L: 235 - 739 (UniProt: P00751)
N, Q: 0 - 85 (UniProt: A0A0H2DUF0)
| PDB | External Database | Details |
|---|---|---|
| Glu 192 | - | expression tag |
| Asn 193 | - | expression tag |
| Leu 194 | - | expression tag |
| Tyr 195 | - | expression tag |
| Phe 196 | - | expression tag |
| Gln 197 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 255 | - | expression tag |
| Glu 470 | - | expression tag |
| Asn 471 | - | expression tag |
| Leu 472 | - | expression tag |
| Tyr 473 | - | expression tag |
| Phe 474 | - | expression tag |
| Gln 475 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 254 | Asp 279 | conflict |
| Ala 674 | Ser 699 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | Ala 31 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 600526.0 | |
| Branched | Number of molecules | 15 |
| Total formula weight | 6167.8 | |
| Non-Polymers* | Number of molecules | 23 |
| Total formula weight | 3831.9 | |
| All* | Total formula weight | 610525.6 |
