6RU5
human complement C3 in complex with the hC3Nb1 nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-21 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 228.320, 140.720, 95.430 |
| Unit cell angles | 90.00, 100.51, 90.00 |
Refinement procedure
| Resolution | 48.250 - 3.900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a73 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.250 | 3.832 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Number of reflections | 179495 | 5016 |
| <I/σ(I)> | 6.1 | |
| Completeness [%] | 97.3 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 100 mM HEPES pH 7.2, 1.75 % (w/v) PEG8000 |
