6RPR
LEM domain of Emerin mutant T43I in complex with BAF dimer and the Igfold of the lamin A/C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2017-06-10 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 80.200, 81.490, 64.880 |
Unit cell angles | 90.00, 121.37, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.260 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ghd |
RMSD bond length | 0.010 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.740 | 2.340 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.096 | |
Rmeas | 0.106 | |
Rpim | 0.050 | |
Number of reflections | 16448 | 16448 |
<I/σ(I)> | 11.74 | |
Completeness [%] | 98.0 | |
Redundancy | 4.5 | |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 18% PEG 3350, 100 mM Tris Bis pH 5.5, 0.1 M NH4SO4 |