6RMV
The crystal structure of a TRP channel peptide bound to a G protein beta gamma heterodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9754 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.860, 80.432, 113.587 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.520 - 1.940 |
R-factor | 0.1896 |
Rwork | 0.188 |
R-free | 0.22430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xhm |
RMSD bond length | 0.007 |
RMSD bond angle | 1.211 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER (2.7.17) |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.520 | 2.010 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.073 | 1.280 |
Rmeas | 0.087 | 1.510 |
Rpim | 0.046 | 0.795 |
Number of reflections | 31659 | 3044 |
<I/σ(I)> | 13.2 | 1.4 |
Completeness [%] | 99.3 | 98.3 |
Redundancy | 6.5 | 6.7 |
CC(1/2) | 0.999 | 0.528 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291.15 | 0.1 M HEPES sodium pH 7.5, 15% (w/v) polyethylene glycol 4,000, 7.5% (v/v) isopropanol |