6R4U

Crystal structure of the Pri1 subunit of human primase bound to fludarabine triphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97944
Spacegroup name	C 2 2 21
Unit cell lengths	110.830, 117.570, 148.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.125 - 2.200
R-factor	0.1975
Rwork	0.196
R-free	0.22320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rb4
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260_000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.125	2.330
High resolution limit [Å]	2.200	2.200
Number of reflections	49380	7541
<I/σ(I)>	12.91	1.52
Completeness [%]	99.2	95.3
Redundancy	6.5
CC(1/2)	0.998	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	23% PEG3350, 10% ethylene glycol, 200 mM Na/K tartrate