6QNZ
Crystal structure of the site-specific DNA nickase N.BspD6I E418A Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.430, 92.250, 113.800 |
Unit cell angles | 90.00, 105.61, 90.00 |
Refinement procedure
Resolution | 47.110 - 2.450 |
R-factor | 0.2315 |
Rwork | 0.231 |
R-free | 0.24320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ewf |
RMSD bond length | 0.004 |
RMSD bond angle | 0.631 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.110 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.040 | 0.690 |
Number of reflections | 49355 | 5127 |
<I/σ(I)> | 11.52 | 1.78 |
Completeness [%] | 87.8 | 92.1 |
Redundancy | 2.1 | 2.1 |
CC(1/2) | 0.990 | 0.750 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.04 M KH2PO4, 16% (w/v) PEG 8000, 20% glycerol |