6QNZ
Crystal structure of the site-specific DNA nickase N.BspD6I E418A Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.430, 92.250, 113.800 |
| Unit cell angles | 90.00, 105.61, 90.00 |
Refinement procedure
| Resolution | 47.110 - 2.450 |
| R-factor | 0.2315 |
| Rwork | 0.231 |
| R-free | 0.24320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ewf |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.631 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.110 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.040 | 0.690 |
| Number of reflections | 49355 | 5127 |
| <I/σ(I)> | 11.52 | 1.78 |
| Completeness [%] | 87.8 | 92.1 |
| Redundancy | 2.1 | 2.1 |
| CC(1/2) | 0.990 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.04 M KH2PO4, 16% (w/v) PEG 8000, 20% glycerol |
