6QNZ
Crystal structure of the site-specific DNA nickase N.BspD6I E418A Mutant
Summary for 6QNZ
Entry DOI | 10.2210/pdb6qnz/pdb |
Descriptor | Heterodimeric restriction endonuclease R.BspD6I large subunit, GLYCEROL, PHOSPHATE ION, ... (4 entities in total) |
Functional Keywords | nickase, endonuclease, nt.bspd6i, nicking, dna, hydrolase |
Biological source | Bacillus sp. D6 |
Total number of polymer chains | 2 |
Total formula weight | 142289.34 |
Authors | Artyukh, R.I.,Kachalova, G.S.,Yunusova, A.K.,Gabdulkhakov, A.G.,Fatkhullin, B.F.,Atanasov, B.P.,Perevyazova, T.A.,Popov, A.N.,Zheleznaya, L.A. (deposition date: 2019-02-12, release date: 2020-03-04, Last modification date: 2024-01-24) |
Primary citation | Artyukh, R.I.,Kachalova, G.S.,Yunusova, A.K.,Fatkhullin, B.F.,Atanasov, B.P.,Perevyazova, T.A.,Popov, A.N.,Gabdulkhakov, A.G.,Zheleznaya, L.A. The key role of E418 carboxyl group in the formation of Nt.BspD6I nickase active site: Structural and functional properties of Nt.BspD6I E418A mutant. J.Struct.Biol., 210:107508-107508, 2020 Cited by PubMed: 32298813DOI: 10.1016/j.jsb.2020.107508 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.45 Å) |
Structure validation
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