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6QNZ

Crystal structure of the site-specific DNA nickase N.BspD6I E418A Mutant

Summary for 6QNZ
Entry DOI10.2210/pdb6qnz/pdb
DescriptorHeterodimeric restriction endonuclease R.BspD6I large subunit, GLYCEROL, PHOSPHATE ION, ... (4 entities in total)
Functional Keywordsnickase, endonuclease, nt.bspd6i, nicking, dna, hydrolase
Biological sourceBacillus sp. D6
Total number of polymer chains2
Total formula weight142289.34
Authors
Artyukh, R.I.,Kachalova, G.S.,Yunusova, A.K.,Gabdulkhakov, A.G.,Fatkhullin, B.F.,Atanasov, B.P.,Perevyazova, T.A.,Popov, A.N.,Zheleznaya, L.A. (deposition date: 2019-02-12, release date: 2020-03-04, Last modification date: 2024-01-24)
Primary citationArtyukh, R.I.,Kachalova, G.S.,Yunusova, A.K.,Fatkhullin, B.F.,Atanasov, B.P.,Perevyazova, T.A.,Popov, A.N.,Gabdulkhakov, A.G.,Zheleznaya, L.A.
The key role of E418 carboxyl group in the formation of Nt.BspD6I nickase active site: Structural and functional properties of Nt.BspD6I E418A mutant.
J.Struct.Biol., 210:107508-107508, 2020
Cited by
PubMed: 32298813
DOI: 10.1016/j.jsb.2020.107508
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.45 Å)
Structure validation

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